rapid salmonella rsal agar  (Bio-Rad)

Journal: MicrobiologyOpen

Article Title: Heat Treatment and Storage of Frass From Black Soldier Fly Larvae and Yellow Mealworm Production: Compliance With EU Regulation on Microbiological Quality and Safety

doi: 10.1002/mbo3.70020

Figure Lengend Snippet: Schematic representation of experiments (Exp) 1, 2, and 3 for frass samples (BSFL or YM); heat treatment (green), Salmonella spp. inoculation (red), storage (blue), and performed analyses (yellow and gray).

Article Snippet: TVC was determined on Plate Count Agar (PCA, Biokar Diagnostics) after incubation at 30°C for 72 h. Enterobacteriaceae count was assessed on Violet Red Bile Glucose agar (VRBG, Biokar Diagnostics) after incubation at 37°C for 24 h. Enterococcaceae count, more specific Enterococci count, was determined on Kanamycin esculin Azide Agar (KAA, Millipore, Darmstadt, Germany) after 48 h at 37°C, and E. coli count on Tryptone bile‐x Glucuronide Agar (TBX, VWR, Leuven, Belgium) incubated at 44°C for 24 h. For count of aerobic endospores, the primary dilution was submitted to a heat shock (80°C for 10 min) followed by pour‐plating a tenfold dilution series on PCA and incubation at 37°C for 24 h. Salmonella spp. count was determined by spread‐plating 100 μL of a dilution series, prepared from 5 g frass as described above, in twofold on RAPID' Salmonella agar (Bio‐Rad Laboratories) and incubation at 37°C for 24 h. For the enumeration of Salmonella spp. in the inoculated frass samples, the agar was supplemented with kanamycin (50 μ/mL agar).

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